RecombinantHumanACE2/ACEHProtein,hFcTag性能参数,分子别名(Synonyms)ACE2;ACEH;ACE-2表达区间及表达系统(Source)HumanACE2/ACEHProteinisexpressedfromHEK293withhFctagattheC-Terminus.ItcontainsGln18-Ser740.[Accession|Q9BYF1-1]分子量大小(MolecularWeight)TheproteinhasapredictedMWof109.2kDa.Duetoglycosylation,theproteinmigratesto115-130kDabasedonSDS-PAGEresult.Endotoxin)Lessthan1EUperμgbytheLALmethod.纯度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.活性(Activity)ELISAData:ImmobilizedSARS-COV-2SpikeS(B.1.1.529/Omicron)Trimer,HisTagat1μg/ml(100μl/well)ontheplate.DoseresponsecurveforHumanACE2,hFcTagwiththeEC50of13.7ng/mldeterminedbyELISA.制剂(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重构方法(Reconstitution)Centrifugethetubebeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.透明质酸的衍生物可以作为基因传递的载体,或用于疫苗佐剂,增强反应。Recombinant Human GCP-2/CXCL6
透明质酸酶,英文名称Hyaluronidase,一类能够降低体内透明质酸活性的酶的总称,结构上由4个相同亚基构成,每个亚基分子量接近14kDa,该酶是一种糖蛋白,包含5%的甘露糖和2.2%的葡萄糖胺。功能上该酶可随机裂解透明质酸、软骨素和硫酸软骨素中的β-N-乙酰己糖胺-[1→4]糖苷键。常与胶原酶联合使用于降解细胞外基质,从而从组织中分离活细胞。本品来源于牛睾丸,活力值为400–1,000units/mg,合适的pH值为4.5–6.0。产品性质中文别名(ChineseSynonym)玻璃糖醛酸酶;玻璃酸酶英文别名(EnglishSynonym)Hyaluronoglucosaminidase;Hyaluronate4-glycanohydrolaseCAS号(CASNO.)37326-33-3来源(Source)牛睾丸分子量(MolecularWeight)~55kDa类型(Type)I-S外观(Appearance)淡黄色至米黄色至褐色粉末TFLLR酵母重组表达N-糖苷酶F(PNGase F)是一种通过酵母重组表达系统生产的酶。
CD40,alsoknownasTNFRSF5,isa45-50kDatypeItransmembraneglycoproteinmemberoftheTNFreceptorsuperfamily.MaturehumanCD40consistsofa173aminoacid(aa)extracellulardomain,atransmembranedomain,anda62aacytoplasmicdomain.CD40servesmultiplefunctionsinbothhematopoieticandepithelialcancersandisatargetfortumorimmunotherapy.DysregulationofCD40/CD40LigandexpressionandinteractionscontributestotheimmunedeficiencyassociatedwithHIVinfectionandAIDS.Itisalsoimplicatedinthepathologyofmultiplecardiovasculardiseasesincludingatherosclerosis,atherothrombosis,andrestenosis.产品性质别名CD40;CD40Lreceptor;TNFRSF5;CD40antigen;CD40molecule;CDw40;MGC9013;UniprotNo.P25942表达区间及表达系统RecombinantBiotinylatedHumanCD40/TNFRSF5ProteinisexpressedfromHEK293CellswithHistagandAvitagattheC-terminal.ItcontainsGlu21-Arg193.分子量Approximately22.1kDa.Duetoglycosylation,theproteinmigratesto35-40kDabasedonTris-BisPAGEresult.纯度>95%asdeterminedbySDS-PAGEandHPLC.活性
RecombinantBiotinylatedHumanENPP-3Protein,His-AviTag性能参数分子别名(Synonyms)NPP3;E-NPP3;PD-Ibeta表达区间及表达系统(Source)BiotinylatedHumanENPP-3ProteinisexpressedfromHEK293withHistagandAvitagattheN-Terminus.ItcontainsLeu48-Ile875.[Accession|O14638]分子量大小(MolecularWeight)TheproteinhasapredictedMWof99.97kDa.Duetoglycosylation,theproteinmigratesto110-130kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.纯度(Purity)>95%asdeterminedbySDS-PAGEandHPLC.制剂(Formulation)Lyophilizedfrom0.22μmfilteredsolutioninPBS(pH7.4).Normally8%trehaloseisaddedasprotectantbeforelyophilization.重构方法(Reconstitution)Centrifugethetubebeforeopening.Reconstitutingtoaconcentrationmorethan100μg/mlisrecommended.Dissolvethelyophilizedproteinindistilledwater.储存条件Theproductshouldbestoredat-25~-15℃for1yearfromdateofreceipt.2-7days,2~8°Cundersterileconditionsafterreconstitution.3-6months,-85~-65℃understerileconditionsafterreconstitution.跨膜蛋白的跨膜区域的相互作用是连接膜外环境与细胞内环境的重要渠道。
Cas9核酸酶是一种向导RNA(guideRNA,gRNA)引导的核酸内切酶,可催化双链DNA的裂解。NLS-Cas9-EGFP在Cas9核酸酶的基础上进行改造,它在N端包含一个核定位信号(NLS),在C端包含一个EGFP和一个6X(His)序列。当Cas9以NLS序列表达时,Cas9RNP复合物在进入细胞后立即定位到细胞核。不需要体内转录或翻译,提高了效率。此外,与其他系统相比,EGFP标签可作为追踪或分类转染细胞的报告器,通过荧光细胞分选(FACS)富集所需基因组编辑的细胞群。它降低了在基因组编辑应用中与单细胞克隆和基因分型相关的人工和成本。产品特点如下:无DNA:没有外部DNA添加;高切割效率:NLS确保Cas9蛋白高效进入细胞核;低脱靶效应:Cas9核酸酶的瞬时表达提高了切割的特异性;节省时间:无需转录和翻译;减少劳动力:通过基于EGFP的FACS富集细胞群以进行所需的基因组编辑。C端His标签增加了融合蛋白检测方法的选择。可应用于:通过体外DNA切割筛选高效和特异性靶向gRNA。通过电穿孔或注射与特定gRNA结合时的体内基因编辑。通过基于EGFP的FACS富集细胞群以进行所需的基因组编辑。储存条件-25~-15℃保存,有效期1年。全长A2aR蛋白具有天然完整构象,VLP(病毒样颗粒)固有特征可以带来更高的免疫原性。PGLa
泛素是一种小分子蛋白,存在于真核生物中,它在细胞内起到调节蛋白质降解、信号转导、细胞周期控制。Recombinant Human GCP-2/CXCL6
RecombinantBiotinylatedCynomolgusSiglec-10Protein,His-AviTag性能参数分子别名(Synonyms)SLG2;SIGLEC10;MGC126774;PRO940表达区间及表达系统(Source)BiotinylatedCynomolgusSiglec-10ProteinisexpressedfromHEK293withHistagandAvitagattheC-terminus.ItcontainsThr17-Asn552.[Accession|A0A2K5WBX8]分子量大小(MolecularWeight)TheproteinhasapredictedMWof61.71kDa.Duetoglycosylation,theproteinmigratesto72-82kDabasedonSDS-PAGEresult.(Endotoxin)Lessthan1EUperμgbytheLALmethod.纯度(Purity)>95%asdeterminedbySDS-PAGE制剂(Formulation)Suppliedas0.22μmfilteredsolutionin25mMMES,150mMNaCl,0.5MArginine(pH5.0).储存条件Theproductshouldbestoredat-85~-65℃for1yearfromdateofreceipt.Recommendtoaliquottheproteinintosmallerquantitieswhenfirstusedandavoidrepeatedfreeze-thawcycles.Recombinant Human GCP-2/CXCL6